THE DEGRADATION BY RIBONUCLEASE OF SUBSTRATES OTHER THAN RIBONUCLEIC ACID
Open Access
- 1 September 1954
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 2 (5), 387-394
- https://doi.org/10.1177/2.5.387
Abstract
These experiments support the premise which is the basis for cytochemical procedures that employ ribonuclease for the identification of ribonucleic acid, namely, that the substrate specifically degraded by ribonuclease is ribonucleic acid. The reduction in Feulgen stainability of chromatin effected by ribonuclease has been shown to be due to an intracellular deoxyribonuclease, which hydrolyzes intracellular deoxyribonucleic acid only after ribonucleic acid has been removed. The degradation of apurinic acid by ribonuclease can be duplicated by other proteins.Keywords
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