Cytological localization of inverted repeated DNA sequences in Vicia faba
- 1 July 1982
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 85 (3), 453-459
- https://doi.org/10.1007/bf00330368
Abstract
Inverted repeated DNA sequences have been isolated from sheared Vicia faba DNA by hydroxylapatite column chromatography, treated with nuclease S1, tritiated by the nick translation method and hybridized in situ on squashes of Vicia faba root tips. Silver grains appear grouped in a rather limited portion of interphase nuclei and form a sort of band across them. The central regions of metaphase chromosomes are preferentially labeled, labeling being excluded from telomeres, centromeres and secondary constrictions. These results are briefly discussed in relation to those obtained in other species and the functional significance of inverted repeats.This publication has 31 references indexed in Scilit:
- Possible palindromic DNAJournal of Theoretical Biology, 1981
- Distribution of repetitive DNA sequences in Vicia faba chromosomesExperimental Cell Research, 1980
- Specific nucleotide sequences in HeLa cell inverted repeated DNA: Enrichment for sequences found in double-stranded regions of heterogeneous nuclear RNAJournal of Molecular Biology, 1977
- Determination of nucleotide sequences from double-stranded regions of HeLa cell nuclear RNAJournal of Molecular Biology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Inverted repetitive sequences in the human genomeBiochemistry, 1976
- An electron microscope study of the DNA sequence organization of the human genomeJournal of Molecular Biology, 1976
- Palindromic base sequences and replication of eukaryote chromosome endsNature, 1974
- Palindromes in chromosomesJournal of Molecular Biology, 1974
- A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition siteJournal of Molecular Biology, 1970