Further Studies on the Behavior of Mumps Virus in Maitland Type of Tissue Cultures

Abstract

By varying the amount of tissue used and the interval between changes of medium, multiplication of mumps virus was shown to take place for as long as 70 days of incubation in Maitland type of tissue cultures. No differences were noted in the pH or visible tissue growth of infected cultures as compared to uninfected ones. Although the number of tissue fragments which were capable of cell migration when transplanted to plasma was somewhat variable, there was no clear-cut evidence that the presence of the virus had a significant effect on the outgrowth of fibroblasts from the explanted tissues. These results suggest that under the conditions of these experiments cytopathogenic activity on the part of mumps virus was minimal at best.