Microfluidic device as a new platform for immunofluorescent detection of viruses
- 4 October 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 5 (11), 1327-1330
- https://doi.org/10.1039/b509086e
Abstract
A bead-based microfluidic device was developed and demonstrated to achieve rapid and sensitive enzyme-linked immunosorbent assay (ELISA) with quantum dots as the labeling fluorophore for virus detection. In comparison to standard ELISA performed on the same virus, the minimal detectable concentration of the target virus was improved from 360 to 22 ng mL−1, the detection time was shortened from >3.25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3.Keywords
This publication has 13 references indexed in Scilit:
- Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridizationAnalytica Chimica Acta, 2005
- Environmental microbiology-on-a-chip and its future impactsTrends in Biotechnology, 2005
- Quantum Dots as a Novel Immunofluorescent Detection System for Cryptosporidium parvum and Giardia lambliaApplied and Environmental Microbiology, 2004
- Innovative tools for detection of plant pathogenic viruses and bacteriaInternational Microbiology, 2003
- Production and characterization of monoclonal antibodies to a grouper iridovirusJournal of Virological Methods, 2003
- Characterization of a novel ranavirus isolated from grouper Epinephelus tauvinaDiseases of Aquatic Organisms, 2003
- Antigenic characterization of a marine fish iridovirus from grouper, Epinephelus sppJournal of Virological Methods, 2002
- Characterization, pathogenicity and neutralization studies of a nervous necrosis virus isolated from grouper, Epinephelus tauvina, in SingaporeAquaculture, 2002
- Array Biosensor for Simultaneous Identification of Bacterial, Viral, and Protein AnalytesAnalytical Chemistry, 1999
- Semiconductor Nanocrystals as Fluorescent Biological LabelsScience, 1998