Histomorphometry of the Embryonic Avian Growth Plate by Proton Nuclear Magnetic Resonance Microscopy
Open Access
- 1 June 2001
- journal article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 16 (6), 1092-1100
- https://doi.org/10.1359/jbmr.2001.16.6.1092
Abstract
Quantitative nuclear magnetic resonance (NMR) microscopy was used to characterize the biochemical and morphological properties of the different zones within the growth plate of an embryonic chick femur. For precalcified tissue, water proton transverse relaxation times (T2) and magnetization transfer values (MT) were directly and inversely dependent, respectively, on tissue cellularity, defined as the intracellular area per unit area on histological sections. T2 values extrapolated for intra- and extracellular water were 96 ms and 46 ms, respectively. The extracellular T2 was comparable with that measured for mature cartilage. The MT values extrapolated for intra- and extracellular compartments were 0.32 and 0.85, respectively. These values were comparable with those values reported in the literature for cell pellets and for mature cartilage tissue. Thus, cellularity dominated the NMR properties of this immature cartilage tissue. Mineral deposits within calcified cartilage and periosteal bone invoked NMR relaxation processes that were dependent on the inorganic mineral phase. Additionally, collagen molecules present in mineralized zones gave rise to a significant MT effect. These results show the utility of water proton NMR microscopy for assessing both the organic and inorganic phases within mineralized tissues.Keywords
This publication has 31 references indexed in Scilit:
- Developing epiphysis: MR imaging characteristics and histologic correlation in the newborn lamb.Radiology, 1998
- Spatial variation of T2 in human articular cartilage.Radiology, 1997
- New Aspects of Endochondral Ossification in the Chick: Chondrocyte Apoptosis, Bone Formation by Former Chondrocytes, and Acid Phosphatase Activity in the Endochondral Bone MatrixJournal of Bone and Mineral Research, 1997
- Hyaline cartilage: in vivo and in vitro assessment with magnetization transfer imaging.Radiology, 1996
- Hyaline cartilage: truncation artifact as a cause of trilaminar appearance with fat-suppressed three-dimensional spoiled gradient-recalled sequences.Radiology, 1996
- Magnetic Resonance Imaging, Histology, and X-Ray of Three Stages of Damage to the Knees of STR/ORT MiceInvestigative Radiology, 1996
- High Resolution Magnetic Resonance Imaging of the Proximal Interphalangeal JointsJournal of Hand Surgery (European Volume), 1996
- Effects of collagen orientation on MR imaging characteristics of bovine articular cartilage.Radiology, 1993
- Cartilaginous epiphysis and growth plate: normal and abnormal MR imaging findings.American Journal of Roentgenology, 1992
- High signal intensity in MR images of calcified brain tissue.Radiology, 1991