CRITICAL REMARKS ON THE DETERMINATION OF URINARY EXCRETION OF ASCORBIC ACID

Abstract
Ascorbic acid in urine cannot be detd. reliably by direct titration with 2, 6 dichlorophenol indophenol. Reducing substances other than ascorbic acid, present in urine, also decolorize this indicator. They can be removed by precipitation with mercuric acetate. The excretion of these interfering substances can increase considerably under various conditions and independently of that of the vit. The technique and significance of saturation tests have been discussed.