Distribution of Glut1 Glucose Transporters in Different Brain Structures Compared to Glucose Utilization and Capillary Density of Adult Rat Brains
Open Access
- 1 February 1997
- journal article
- research article
- Published by SAGE Publications in Journal of Cerebral Blood Flow & Metabolism
- Vol. 17 (2), 204-209
- https://doi.org/10.1097/00004647-199702000-00010
Abstract
Glut1 is a specific transporter system that mediates glucose transfer across the blood–brain barrier (BBB). Although the main location of Glut1 is in the capillary endothelium of the brain, its local distribution in different brain regions is not as well defined. In the present investigation, the local pattern of Glut1 distribution was determined in 13 brain structures using an immunoautoradiographic method developed for this purpose. A polyclonal antibody directed against the C-terminal amino acid sequence of Glut1 was applied to cryosections of rat brains. A secondary antibody was added that had been coupled to [35S]. Results show a heterogeneous distribution of Glut1 in the brain with activities of [35S] ranging from 65% below to 15% above the mean. White matter activity was lower than gray matter activity. For comparison, capillary sections were counted in corresponding cryosections by indirect immunofluorescence using fibronectin antibodies. In addition, local cerebral glucose utilization (LCGU) was analyzed in identical brain structures of conscious rats by the quantitative autoradiographic 2-deoxyglucose method. Significant correlations were found between Glut1 density and either LCGU or capillary density. Results indicate a tight coupling of Glut1 transporter density and capillary density to the LCGU of different BBB structures in adult rats.Keywords
This publication has 21 references indexed in Scilit:
- Regional Congruence and Divergence of Glucose Transporters (GLUT1) and Capillaries in Rat BrainsJournal of Cerebral Blood Flow & Metabolism, 1995
- The human blood‐brain barrier glucose transporter (GLUT1) is a glucose transporter of gray matter astrocytesGlia, 1995
- Differential glycosylation of the GLUT1 glucose transporter in brain capillaries and choroid plexusBiochimica et Biophysica Acta (BBA) - Biomembranes, 1994
- Developmental Expression of GLUT1 and GLUT3 Glucose Transporters in Rat BrainJournal of Neurochemistry, 1994
- Immunological Analysis of Glucose Transporters Expressed in Different Regions of the Rat Brain and Central Nervous SystemBiochemical and Biophysical Research Communications, 1993
- Glucose Transporter Isoforms in Brain: Absence of GLUT3 from the Blood—Brain BarrierJournal of Cerebral Blood Flow & Metabolism, 1993
- Ontogeny and cellular distribution of brain glucose transporter gene expressionMolecular and Cellular Neuroscience, 1992
- Expression of mouse-GLUT3 and human-GLUT3 glucose transporter proteins in brainBiochemical and Biophysical Research Communications, 1992
- Glucose transporter localization in brain using light and electron immunocytochemistryJournal of Neuroscience Research, 1989
- THE [14C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT1Journal of Neurochemistry, 1977