Determination of L-erythro-Tetrahydrobiopterin in Biological Tissues by High Pressure Liquid Chromatography and Electrochemical Detection

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Abstract
L-erythro-Tetrahydrobiopterin (H4-biopterin) was determined in rat tissue (liver, corpus striatum, brain stem) by reversed phase high pressure liquid chromatography and subsequent electrochemical detection. Due to the low oxidation potential (+ 250 mV) necessary for the analysis of H4 biopterin, the electrochemical detector is a highly selective system for this compound. For analysis of H4 biopterin in biological tissues, only 1 extraction step with 0.2 M perchloric acid and 10 mM ascorbic acid is necessary prior to analysis. The H4 biopterin content of rat tissue determined with this method corresponds well with the values reported elsewhere.