Immunocharacterization of δ‐ and ζ‐isoenzymes of protein kinase C in rat renal mesangial cells
- 6 April 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 300 (3), 259-262
- https://doi.org/10.1016/0014-5793(92)80858-e
Abstract
The isoforms of protein kinase C (PKC) present in rat mesangial cells were identified by immunoblot analysis with antibody raised against isotype-specific peptides. In addition to the previously observed α- and ε-subspecies, mesangial cells also express the δ- and ζ-isoenzymes of PKC. On exposure to phorbol 12,13-dibutyrate (PDB) a complete depletion of PKC-δ is observed within 8 h. Removal of PDB results in a recovery of PKC-δ. In contrast, PKC-ζ is unaffected by addition or removal of PDB.Keywords
This publication has 27 references indexed in Scilit:
- Structure and properties of a ubiquitously expressed protein kinase C, nPKCδEuropean Journal of Biochemistry, 1991
- Differential recovery of protein kinase C-α and -ɛ isozymes after long-term phorbol ester treatment in rat renal mesangial cellsBiochemical and Biophysical Research Communications, 1991
- Expression and characterization of protein kinase C‐δEuropean Journal of Biochemistry, 1991
- Different translocation of three distinct PKC isoforms with tumor-promoting phorbol ester in human plateletsBiochemical and Biophysical Research Communications, 1991
- Transforming growth factor β2 inhibits interleukin 1β- and tumour necrosis factor α-induction of nitric oxide synthase in rat renal mesangial cellsBiochemical and Biophysical Research Communications, 1991
- Regulatory functions of protein kinase c in glomerular mesangial cellsKlinische Wochenschrift, 1990
- ζ-Related protein kinase C in nuclei of nerve cellsBiochemical and Biophysical Research Communications, 1990
- Differentiative action of K252a on protein kinase C and A calcium-unresponsive, phorbol ester/phospholipid-activated protein kinaseBiochemical and Biophysical Research Communications, 1989
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970