Localization of creatine kinase isoenzymes in myofibrils. II. Chicken heart muscle.
Open Access
- 1 November 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 75 (2), 318-325
- https://doi.org/10.1083/jcb.75.2.318
Abstract
Chicken heart muscle contains almost exclusively the BB isoenzyme of creatine kinase (CK), its myofibrils, moreover, lack an M-line. This tissue thus provides an interesting contrast to skeletal muscle, in which some of the MM-CK present as predominant CK isoenzyme is bound at the myofibrillar M-line. Approx. 2% of the total CK activity in a chicken heart homogenate remains bound to the myofibrillar fraction after repeated washing cycles; both the fraction and the absolute amount of CK bound are about threefold lower than in skeletal muscle. Almost all of the bound enzyme is located within the Z-line region of each sarcomere, as revealed by indirect fluorescent-antibody staining with antiserum against purified chicken BB-CK. After incubation with exogenous purified MM-CK, positive immunofluorescent staining for M-type CK at the H-region of heart myofibrils was observed, along with weaker fluorescence in the Z-line region. Chicken heart myofibrils may thus possess binding sites for both M and B forms of CK.This publication has 11 references indexed in Scilit:
- Compositional studies of myofibrils from rabbit striated muscle.The Journal of cell biology, 1976
- Structure of muscle filaments from immunohistochemical and ultrastructural studies.Journal of Histochemistry & Cytochemistry, 1975
- Removal of Z-lines and alpha-actinin from isolated myofibrils by a calcium-activated neutral proteaseJournal of Biological Chemistry, 1975
- Creatine kinase of rat heart mitochondria. Coupling of creatine phosphorylation to electron transport.1973
- THE ULTRASTRUCTURE OF Z DISKS FROM WHITE, INTERMEDIATE, AND RED FIBERS OF MAMMALIAN STRIATED MUSCLESThe Journal of cell biology, 1973
- The ultrastructure of ordinary and Purkinje cells of the fowl heart.1971
- Binding of Aldolase and Triosephosphate Dehydrogenase to F‐Actin and Modification of Catalytic Properties of AldolaseEuropean Journal of Biochemistry, 1970
- QUANTITATIVE AND HISTOCHEMICAL STUDIES ON THE DESORPTION AND READSORPTION OF ALDOLASE IN CROSS-STRIATED MUSCLEJournal of Histochemistry & Cytochemistry, 1969
- Cardiac muscle. A comparative ultrastructural study with special reference to frog and chicken hearts.1969
- THE ULTRASTRUCTURE OF THE Z DISC IN SKELETAL MUSCLEThe Journal of cell biology, 1962