Laminin promotes rabbit neutrophil motility and attachment.
Open Access
- 1 April 1986
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 77 (4), 1180-1186
- https://doi.org/10.1172/jci112419
Abstract
Polymorphonuclear neutrophils (PMN) traverse basement membrane to reach sites of infection. We have studied the role of laminin, a specific basement membrane component, in this process using three assay systems. In the Boyden chamber, laminin was found to stimulate chemotaxis of neutrophils while fibronectin did not. Co-incubation of cells with antibody to laminin blocked this chemotaxis, while antibody to fibronectin was without effect. In the human amnion system, neutrophils were shown to penetrate through the tissue when the peptide chemoattractant f-Met-Leu-Phe was placed on the opposing side. Antibody to laminin, but not to fibronectin, blocked this penetration. In an attachment assay system, laminin, but not fibronectin, was found to increase dispase-treated neutrophil attachment to type IV (basement membrane) collagen-coated plastic and to a plastic substrate itself. Electrophoretic analysis of PMN extract indicated the presence of laminin, and indirect immunofluorescence suggested that laminin is localized on the surface of the neutrophils. These data suggest that PMN can bind laminin on their cell surfaces, use laminin to attach to basement (type IV) membrane collagen, and migrate toward a gradient of laminin. These properties may be important for the passage of neutrophils from the circulation to sites of infection.This publication has 31 references indexed in Scilit:
- The Regulation of Basement Membrane Formation and Cell‐Matrix Interactions by Defined Supramolecular ComplexesPublished by Wiley ,1984
- SUBSTANCES WHICH AGGREGATE NEUTROPHILS - MECHANISM OF ACTION1978
- Binding of soluble form of fibroblast surface protein, fibronectin, to collagenInternational Journal of Cancer, 1977
- The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils.The Journal of Experimental Medicine, 1976
- N-formylmethionyl peptides as chemoattractants for leucocytes.Proceedings of the National Academy of Sciences, 1975
- Nature of the collagen synthesized by a transplanted chondrosarcomaArchives of Biochemistry and Biophysics, 1975
- Isolation of a collagen-dependent cell attachment factorNature, 1974
- Short-Term Binding and Hydrolysis of Cyclic 3′:5′-Adenosine Monophosphate by Aggregating Dictyostelium CellsProceedings of the National Academy of Sciences, 1974
- (Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life.1974
- The Nature of the Intramolecular Cross-Links in Collagen. The Separation and Characterization of Peptides from the Cross-Link Region of Rat Skin Collagen*Biochemistry, 1966