Human xeroderma pigmentosum group G gene encodes a DNA endonuclease
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (16), 3312-3316
- https://doi.org/10.1093/nar/22.16.3312
Abstract
Because of defective nucleotide excision repair of ultraviolet damaged DNA, xeroderma pigmentosum (XP) patients suffer from a high incidence of skin cancers. Cell fusion studies have identified seven XP complementation groups, A to G. Previous studies have implicated the products of these seven XP genes in the recognition of ultraviolet-induced DNA damage and in incision of the damage-containing DNA strand. Here, we express the XPG-encoded protein in Sf9 insect cells and purify it to homogeneity. We demonstrate that XPG is a single-strand specific DNA endonuclease, thus identifying the catalytic role of the protein in nucleotide excision repair. We suggest that XPG nuclease acts on the single-stranded region created as a result of the combined action of the XPB helicase and XPD helicase at the DNA damage site.Keywords
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