HUMAN SEMEN RIBONUCLEASE - LOCATION, PROPERTIES AND INHIBITION BY SODIUM DODECYL-SULFATE, ZINC-SULFATE AND EDTA
- 1 January 1976
- journal article
- research article
- Vol. 21 (2), 109-113
Abstract
Optimal conditions were established for RNase activity measurement. The enzyme was measured in human seminal plasma as well as in spermatozoa. Sperm enzyme may come from seminal plasma contamination and that RNase may be used as a marker enzyme for seminal plasma contamination. Na dodecyl sulfate, a reagent utilized to produce the solubilization of the spermatozoa, produced a very strong inhibition of the RNase at low concentrations (530 .mu.M). Zinc sulfate and EDTA also produced inhibition of the RNase activity. Such inhibition may be very useful in future studies of RNA metabolism in human spermatozoa.This publication has 5 references indexed in Scilit:
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