The corrinoid from Methanobacterium thermoautotrophicum (Marburg strain). Spectroscopic structure analysis and identification as Cobeta-cyano-5'-hydroxybenzimidazolyl-cobamide (factor III)
- 1 January 1987
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 162 (2), 275-278
- https://doi.org/10.1111/j.1432-1033.1987.tb10596.x
Abstract
The corrinoids from Methanobacterium thermoautotrophicum were extracted as the Co-cyano derivative, which was isolated in crystalline form. A consistent set of spectroscopic data was acquired (ultraviolet/visible, circular dichroic, infrared, fast-atom-bombardment mass, 1H-NMR and 13C-NMR spectra), which allowed the structural analysis of this complete corrinoid. It was assigned the structure of the Co.beta.-cyano-5''-hydroxybenzimidazolyl-cobamide and was identified with Friedrich and Bernhauer''s ''factor III'' by comparison with an authentic sample.This publication has 36 references indexed in Scilit:
- Carbon assimilation pathways in archaebacteriaSystematic and Applied Microbiology, 1986
- REVIEWBiological Chemistry Hoppe-Seyler, 1985
- Acetyl‐cobalamin from Photoinduced Carbonylation of Methyl‐cobalaminHelvetica Chimica Acta, 1984
- Purification and analysis of cobamides of Methanobacterium bryantii by high-performance liquid chromatographyAnalytical Biochemistry, 1984
- The β-ligandsBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Isolation and characterization of the Co‐methyl and Co‐aquo derivative of 5‐hydroxybenzimidazolylcobamide (factor III) from Methanosarcina barkeri grown on methanolFEBS Letters, 1983
- Corrinoids from Methanosarcina barkeri: Structure of the α-ligandBiochemical and Biophysical Research Communications, 1982
- Effect of trace elements and vitamins on the growth of Methanosarcina barkeriActa Biotechnologica, 1981
- Quantification of corrinoids in methanogenic bacteriaCurrent Microbiology, 1980
- Corrinoid Compounds of Methanobacillus omelianskii. I. Fractionation of the Corrinoid Compounds and Identification of Factor III and Factor III Coenzyme*Biochemistry, 1965