Determination of Flecainide in Human Plasma by High Performance Liquid Chromatography with Fluorescence Detection

Abstract
A simple, rapid, selective and sensitive high-performance liquid chromatographic (HPLC) method for the monitoring of plasma flecainide [an antiarrhythmic agent] levels in a therapeutic or research environment is described. The drug is first separated from plasma by a single-step extraction with hexane and then quantitated by HPLC with fluorescence detection. Two linear ranges were established, i.e., 100-2000 ng/ml for drug monitoring in clinical management of patients and 3-300 ng/ml for pharmacokinetic studies. The intra-day variation is less than 6%.