Differential Response of Cultured Parsley Cells to Elicitors from Two Non-pathogenic Strains of Fungi. 1. Identification of Induced Products as Coumarin Derivatives
Open Access
- 1 March 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 131 (2), 401-407
- https://doi.org/10.1111/j.1432-1033.1983.tb07277.x
Abstract
Dark-grown cell suspension cultures of parsley, Petroselinum hortense, produce furanocoumarins after treatment with elicitor preparations of either Phytophthora megasperma f.sp. glycinea (Pmg elicitor) or Alternaria carthami Chowdhury (Ac elicitor). The linear furanocoumarins, psoralen and xanthotoxin, and the benzodipyrandione, graveolone, are the major products synthesized in response to Pmg elicitor, besides small amounts of the furanocomarin bergapten. Treatment with Ac elicitor induces predominantly the formation of bergapten and the furanocoumarin isopimpinellin, as well as small amounts of graveolone. While Pmg elicitor leads to cell death within a few days, cell mass increased for at least 6 days after treatment with Ac elicitor. Brefeldin A, a phytotoxin produced by A. carthami, inhibits growth of parsley cell suspension cultures considerably at a concentration of 0.01 mM and growth of the cells ceased at a concentration of 0.1 mM toxin. Concomitantly, furanocoumarin biosynthesis was suppressed in our system by a concentration of brefeldin A within 0.01–0.1 mM.This publication has 28 references indexed in Scilit:
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