THE BOVINE LYMPHOID SYSTEM .3. A MONOCLONAL-ANTIBODY SPECIFIC FOR BOVINE CELL-SURFACE AND SERUM IGM
- 1 January 1980
- journal article
- research article
- Vol. 40 (3), 359-365
Abstract
Mouse spleen cells from animals immunized with bovine peripheral blood lymphocytes were fused to [mouse] X63.Ag8 myeloma cells, the activity of one of the resulting myeloma hybrids was characterized. The product of this clone (B5/4.1.4) binds to pentameric bovine Ig[immunoglobulin]M isolated from serum, butnot to serum IgG1 or IgG2. This reagent also binds to cell surface (monomeric) IgM and can be used in immunofluorescence assays to enumerate IgM-bearing cells in lymphoid cell suspensions and to examine B [bone marrow] lymphocytes or B lymphocyte-derived cells in tissue sections.This publication has 11 references indexed in Scilit:
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