ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.
- 1 March 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (3), 1365-1368
- https://doi.org/10.1073/pnas.77.3.1365
Abstract
The heat-stable polypeptide (APF-1) required for ATP-dependent proteolysis in [rabbit] reticulocytes enters into high MW conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE-cellulose. Conjugate formation requires ATP and Mg2+ and is inhibited by N-ethylmaleimide. UTP and GTP are inactive. These properties are identical to those of ATP-dependent protein breakdown in the same system, suggesting that the conjugates are intermediates in this process. The APF-1 conjugates are stable in sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid, alkali, heat denaturation and reduction; the conjugates are therefore covalent.This publication has 16 references indexed in Scilit:
- A heat-stable polypeptide component of an ATP-dependent proteolytic system from reticulocytesBiochemical and Biophysical Research Communications, 1978
- Intermediate steps in the degradation of a specific abnormal protein in Escherichia coliJournal of Biological Chemistry, 1977
- Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.Proceedings of the National Academy of Sciences, 1977
- A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.Proceedings of the National Academy of Sciences, 1977
- Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cellsBiochemistry, 1975
- Influence of protease inhibitors and energy metabolism on intracellular protein breakdown in starving Escherichia coliBiochemical and Biophysical Research Communications, 1973
- Protein degradation in cultured cells. The effect of fresh medium, fluoride, and iodoacetate on the digestion of cellular protein of rat fibroblasts.1973
- Turnover of Intracellular ProteinsAnnual Review of Microbiology, 1972
- Studies on the degradation of tyrosine aminotransferase in hepatoma cells in culture. Influence of the composition of the medium and adenosine triphosphate dependence.1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970