Histology and histochemistry of elongating needles of Pinus strobus subjected to a long-duration, low-concentration exposure of sulfur dioxide

Abstract

Two-year-old seedlings of Pinus strobus were grown from budbreak to bud set (11 weeks) in air containing 132 ± 26 μg SO₂∙m⁻³. Histological and histochemical effects on elongating needles were examined. Cellular injury was restricted to mesophyll parenchyma. Affected cells manifested a progressive alteration of protoplast staining proportional to the degree of injury. Total carbohydrates and plastids aggregated at cell walls. Total proteins and proteins containing sulfhydryl–disulfide groups decreased. Phospholipid staining in the plasmalemma – cell wall region appeared reduced. Succinic dehydrogenase activity was enhanced and was apparent longer in injured cells. Needle ontogeny was slowed in fumigated seedlings. There were no significant differences in external growth parameters after 11 weeks. The injury can, therefore, be classified as latent or hidden. The SO₂ effects could contribute to a growth reduction in successive increments.