Is there left-handed DNA at the ends of yeast chromosomes?
- 1 March 1983
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 302 (5903), 84-86
- https://doi.org/10.1038/302084a0
Abstract
Tracts of the alternating copolymer poly(dGdT · dCdA) have been observed in a variety of eukaryotes1. Such tracts are of particular interest since homopolymers of this sequence can exist in vitro as left-handed Z form DNA2. We have found that the yeast Saccharomyces cerevisiae contains at least 30 poly(GT) tracts at dispersed genomic locations. We show here that one subset of these tracts is located at the ends (telomeres) of the yeast chromosome. In addition, we show that poly(dGdT·dCdA) tracts are added to the ends of the extra-chromosomal ribosomal DNA molecules of Tetrahymena when cloned in yeast. These data represent the first reported association between a homopolymeric sequence and a chromosome structure.Keywords
This publication has 11 references indexed in Scilit:
- Potential Z-DNA forming sequences are highly dispersed in the human genomeNature, 1982
- The Three-Dimensional Structure of DNAAnnual Review of Biochemistry, 1982
- Cloning yeast telomeres on linear plasmid vectorsCell, 1982
- A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human δ and β globin genesNucleic Acids Research, 1981
- Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase.Proceedings of the National Academy of Sciences, 1980
- A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in TetrahymenaJournal of Molecular Biology, 1978
- A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteumJournal of Molecular Biology, 1978
- Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAsBiochemistry, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Chapter 16 The Use of Fluorescent DNA-Binding Agent for Detecting and Separating Yeast Mitochondrial DNAMethods in Cell Biology, 1975