Purification of IAA from Shoot Tissues2Phaseolus vulgarisand its Analysis by GC-MS
- 1 April 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 29 (2), 375-386
- https://doi.org/10.1093/jxb/29.2.375
Abstract
Methods are described for the analysis of indole-3-acetic acid (IAA) in extracts from shoot tissues of Phaseolus vulgaris. The purification procedures were designed to supply samples of low dry weight suitable for identification and quantitative estimations by combined gas chromatography-mass spectrometry (GC-MS). Three methods of obtaining stable IAA derivatives for GLC were assessed. For quantitative studies, the losses incurred during the purification stages were gauged by radio-GLC, and a selective ion detection (SID) method was used to calculate amounts present in derivatized samples.This publication has 4 references indexed in Scilit:
- Apical dominance and the levels of indole acetic acid in Phaseolus lateral budsPlanta, 1977
- The involvement of indole acetic acid in the thermodormancy of lettuce fruits, Lactuca sativa cv. Grand RapidsPlanta, 1976
- An improvement of auxin extraction procedure and its application to cultured plant cellsPlanta, 1976
- Characterization of plant growth substances in Citrus unshiu and their change in fruit developmentPlant and Cell Physiology, 1975