In Vivo Methods to Study Uptake of Nanoparticles into the Brain
Open Access
- 7 October 2010
- journal article
- research article
- Published by Springer Nature in Pharmaceutical Research
- Vol. 28 (3), 456-471
- https://doi.org/10.1007/s11095-010-0291-7
Abstract
Several in vivo techniques have been developed to study and measure the uptake of CNS compounds into the brain. With these techniques, various parameters can be determined after drug administration, including the blood-to-brain influx constant (Kin), the permeability-surface area (PS) product, and the brain uptake index (BUI). These techniques have been mostly used for drugs that are expected to enter the brain via transmembrane diffusion or by carrier-mediated transcytosis. Drugs that have limitations in entering the brain via such pathways have been encapsulated in nanoparticles (based on lipids or synthetic polymers) to enhance brain uptake. Nanoparticles are different from CNS compounds in size, composition and uptake mechanisms. This has led to different methods and approaches to study brain uptake in vivo. Here we discuss the techniques generally used to measure nanoparticle uptake in addition to the techniques used for CNS compounds. Techniques include visualization methods, behavioral tests, and quantitative methods.Keywords
This publication has 103 references indexed in Scilit:
- Visualization of exogenous delivery of nanoformulated butyrylcholinesterase to the central nervous systemChemico-Biological Interactions, 2010
- Evaluation of [11C]-DAA1106 for imaging and quantification of neuroinflammation in a rat model of herpes encephalitisNuclear Medicine and Biology, 2010
- Transport across the Blood-Brain Barrier of Pluronic LeptinJournal of Pharmacology and Experimental Therapeutics, 2010
- Structure and function of the blood–brain barrierNeurobiology of Disease, 2009
- Evaluation of O-[18F]fluoromethyl-d-tyrosine as a radiotracer for tumor imaging with positron emission tomographyNuclear Medicine and Biology, 2009
- Developing drugs that can cross the blood-brain barrier: applications to Alzheimer's diseaseBMC Neuroscience, 2008
- Antitumour activity of ANG1005, a conjugate between paclitaxel and the new brain delivery vector Angiopep‐2British Journal of Pharmacology, 2008
- TAT-conjugated nanoparticles for the CNS delivery of anti-HIV drugsBiomaterials, 2008
- Memantine leads to behavioral improvement and amyloid reduction in Alzheimer's‐disease‐model transgenic mice shown as by micromagnetic resonance imagingJournal of Neuroscience Research, 2008
- Brain Uptake Kinetics of Nicotine and Cotinine after Chronic Nicotine ExposureJournal of Pharmacology and Experimental Therapeutics, 2005