Transformation in vitro of the Embryonal Hamster Brain Cells by Human Adenovirus Type 12

Abstract

The cells obtained from 15 to 19 day old embryonal brain tissues of random bred strains of golden hamsters (Mesocrisetus auratus) were inoculated with high titer adenovirus type 12 (Huie strain). In 20 days foci of transformed cells appeared in the cultures when inoculated with more than 106 TCID50/ml of virus. They were circumscribed by the surrounding cells and remained adherent to one another. Three of these foci had adenovirus type specific T antigens. Four cell lines obtained from each focus were examined for their tumor producing capacity. All the lines tested were highly malignant.