Studies on the Elimination of the Complement Inhibiting Activity of Human γ-Globulin Using Degradation with Plasmin or Gel Filtration
- 1 January 1967
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 31 (4), 380-389
- https://doi.org/10.1159/000229883
Abstract
Plasmin degrades gammaG-globulin very similarly to trypsin. Plasmin degrades the polymers in gamma-globulin preparations, but it simultaneously fragments the monomer gamma- globulin even in low concentration. Therefore it is not ideal for the preparation of a polymer free gamma-globulin that does not fix complement. By gel filtration through Sephadex G-200, the aggregates are separated from the mono-mer 7 S gamma-globulin which is not anticomplementary. This mono-mer fraction soon gives rise to new C[image]-fixing di- and polymers of the gammaG- globulin.This publication has 12 references indexed in Scilit:
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