Lanthanoid(III) cations as nuclear magnetic resonance conformational probes: studies on cytidine 5′-monophosphate at pH 2

Abstract

Complex formation between cytidine 5′-monophosphate (cmp) and lanthanoid(III) cations has been followed by n.m.r. spectroscopy, making use of induced shifts of the n.m.r. lines of cmp caused by the bound lanthanoid cations. Shift data have been obtained under a variety of conditions using most of the lanthanoids. These data show that the conformation of cmp is similar to that of adenosine 5′-monophosphate (amp). Relaxation studies employing Gd^III as perturbing cation confirm the conclusions drawn from the shift data.