G-6-PD Manchester: A New Variant Associated With Chronic Nonspherocytic Hemolytic Anemia

Abstract

A variant of glucose-6-phosphate dehydrogenase (G-6-PD) designated G-6-PD Manchester, and associated with chronic nonspherocytic hemolytic anemia, was found in an English male. The electrophoretic mobility at pH 7.0 of this G-6-PD variant is the slowest yet described. Substrate specificity is normal but enzyme activity is markedly inhibited by NADPH, and this is thought to account for the severe chronic hemolysis.