The Kinetics of the Formation of the Primary Lactoperoxidase – Hydrogen Peroxide Compound

Abstract

The kinetics of the formation of the primary lactoperoxidase – hydrogen peroxide compound (compound I) at 25 °C have been studied over the pH range 3.0–10.8 by steady state methods. The second-order rate constant k₁ is pH-independent over the pH region investigated, having a value of (9.2 ± 0.9) × 10⁶M⁻¹s⁻¹. An anomalous effect of formate buffer on the kinetics of the formation of compound I is reported.