Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.

Abstract

Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus were used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of hybrid-selected mRNA in an in vitro system supplemented with canine pancreatic microsomes permitted VP7 to be assigned to segment 9 and the 2 nonstructural viral proteins NCVP4 and NCVP3, to segments 7 and 8, respectively. Hybridization of cloned DNA probes for segments 7-9 with the corresponding segments of human rotavirus Wa confirmed these assignments. The complete nucleotide sequence of gene 9 was determined. The deduced amino acid sequence reveals VP7 to be 326 amino acids in length with 2 NH2-terminal hydrophobic regions and a single glycosylation site at residues 69-71.