A Rapid, Automated Microtiter Assay for Measuring Neutralization of HIV-1
- 1 August 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 9 (8), 781-785
- https://doi.org/10.1089/aid.1993.9.781
Abstract
A sensitive, rapid, and quantative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.Keywords
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