The Juvenile Hormones in Blood of Larvae and Adults of Manduca sexta (Joh.)

Abstract

The juvenile hormones (JH) in extracts of M. sexta blood were converted to 10-(2,4-dichlorobenzoyloxy)-11-methoxy derivatives and quantitatively determined by gas chromatography with electron capture detection. Early IVth instar larvae contained 0.62 ng JH-I, 1.1 ng JH-II and 0.07 ng JH-III per ml blood. Early Vth instar larvae, and adult of both sexes contained the hormones in concentrations from 0-0.24 ng/ml blood. No hormone was detected in late Vth instar (wandering) larvae. The detection limit in these experiments was .ltoreq. 0.003 ng/ml blood.