The membrane-associated F0F1ATPase is essential for the viability ofStreptococcus pneumoniae
Open Access
- 1 June 2002
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 212 (1), 133-138
- https://doi.org/10.1111/j.1574-6968.2002.tb11256.x
Abstract
Genetic studies aimed at eliminating expression of the atp operon (F0F1 H+-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.Keywords
This publication has 24 references indexed in Scilit:
- Genome of the Bacterium Streptococcus pneumoniae Strain R6Journal of Bacteriology, 2001
- Optochin Resistance inStreptococcus pneumoniae:Mechanism, Significance, and Clinical ImplicationsThe Journal of Infectious Diseases, 2001
- The promoter of the operon encoding the F0F1 ATPase of Streptococcus pneumoniae is inducible by pHMolecular Microbiology, 2001
- Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniaeScience, 2001
- The epidemiology of pneumococcal infection in children in the developing worldPhilosophical Transactions Of The Royal Society B-Biological Sciences, 1999
- Quinine specifically inhibits the proteolipid subunit of the F0F1 H+ -ATPase of Streptococcus pneumoniaeJournal of Bacteriology, 1996
- The Prevalence of Drug-ResistantStreptococcus pneumoniaeIn AtlantaNew England Journal of Medicine, 1995
- Resistance to Penicillin and Cephalosporin and Mortality from Severe Pneumococcal Pneumonia in Barcelona, SpainNew England Journal of Medicine, 1995
- Molecular basis of the optochin‐sensitive phenotype of pneumococcus: characterization of the genes encoding the F0 complex of the Streptococcus pneumoniaeStreptococcus oralis H+‐ATPasesMolecular Microbiology, 1994
- ATP SYNTHASE (H+-ATPase): Results by Combined Biochemical and Molecular Biological ApproachesAnnual Review of Biochemistry, 1989