Results Following Hypothermic Asanguineous Liver Perfusion in Dogs

Abstract

An experimental model was developed to study the effects of asanguineous hypothermic liver perfusion in the intact dog. Experimental groups were varied in relation to duration of perfusion, depth of hypothermia and composition of the perfusate. Long-term survival (resumption of normal hepatocellular function) was observed in 86% (90-min. perfusion), 72% (120-min. perfusion) and 66% (150-min. perfusion). Hypotension and congestion following revascularization did not occur in most instances of prolonged perfusions. Metabolic and microscopic ilterations were studied. Optimal hepatic preservation can be best achieved by continuous perfusion of the liver during the anoxic interval. This technique allows the regulation of tissue pH with buffering agents, maintenance of optimal levels of hypothermia (10 to 15[degree]C.) and prevention of accumulation of acid metabolites.