A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis‐complex pathogens
- 11 March 1991
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 70 (3), 221-226
- https://doi.org/10.1111/j.1365-2672.1991.tb02928.x
Abstract
A 1.5 kb EcoRI-BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculous infections.Keywords
This publication has 31 references indexed in Scilit:
- Polymerase Chain Reaction for the Detection of Mycobacterium lepraeMicrobiology, 1989
- Detection and identification of mycobacteria by amplification of mycobacterial DNAMolecular Microbiology, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear CellsScience, 1988
- BCG Identification by DNA Restriction Fragment PatternsMicrobiology, 1987
- Mycobacteria Other than Mycobacterium tuberculosis: Review of Microbiologic and Clinical AspectsClinical Infectious Diseases, 1987
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- A Review of the Methods of Identification and Differentiation of MycobacteriaClinical Infectious Diseases, 1981
- A Relatively Rapid Procedure for the Preparation of Lysis-susceptible Forms of Mycobacterium smegmatisJournal of General Microbiology, 1978
- A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genesBiochemistry, 1977