Quisqualate and L-glutamate inhibit retinal horizontal-cell responses to kainate.

Abstract

Currents elicited by L-Glu and the related agonists quisqualate and kainate were analyzed under voltage clamp in isolated goldfish horizontal cells, using the whole-cell recording configuration of the patch-clamp method. These currents resulted from an increase in cationic conductance and were indistinguishable from one another in terms of reversal potential (.apprxeq. 0 mV) and apparent elementary conductance (2-3pS [picosiemens]). The power-density spectra of the noise increases produced by each agonist were fit by the sum of 2 Lorentzian curves having similar cutoff frequencies (.tau.1 .simeq. 5 ms, .tau.2 .simeq. 1 ms), but the relative power of these components were different for quisqualate and Glu than for kainate. The responses to high doses of either quisqualate or Glu rapidly faded, whereas the response to kainate did not. Finally, quisqualate and Glu produced an inhibition of responses to kainate which appeared to be uncompetitive. Kainate, quisqualate and in this preparation, Glu appear to activate channels different than those activated by N-methyl-D-Asp in other preparations. At least some of the effects of quisqualate and Glu appear to be mediated by receptors bound by kainate.