Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis
- 1 January 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 193 (1), 371-374
- https://doi.org/10.1042/bj1930371
Abstract
Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.This publication has 10 references indexed in Scilit:
- Double-labeling and high precision comparison of complex protein patterns on two-dimensional polyacrylamide gelsAnalytical Biochemistry, 1980
- Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuriaBiochemical Journal, 1979
- Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresisAnalytical Biochemistry, 1979
- The tritlum labeling of small amounts of protein for analysis by electrophoresis on sodium dodecyl sulfate-polyacrylamide slab gelsAnalytical Biochemistry, 1979
- Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydrideAnalytical Biochemistry, 1978
- High resolution two-dimensional electrophoresis of proteins.1975
- A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide GelsEuropean Journal of Biochemistry, 1974
- Radioactive Labeling of Proteins in VitroJournal of Biological Chemistry, 1971
- Reductive alkylation of amino groups in proteinsBiochemistry, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951