Tyrosine Hydroxylase mRNA Concentration in Midbrain Dopaminergic Neurons Is Differentially Regulated by Reserpine

Abstract
Tyrosine hydroxylase (TH)‐mRNA, assayed by in situ hybridization combined with TH immunocytochemistry, showed a selective increase in the ventral tegmental area (A‐10) but not in the substantia nigra (A‐9) midbrain dopaminergic (DAergic) neurons 3 days after reserpine treatment. TH‐mRNA in locus ceruleus noradrenergic (A‐4) neurons was increased by reserpine, as confirmed by RNA blot hybridization. These findings show that TH‐mRNA is differentially regulated in midbrain DAergic neurons in response to reserpine.

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