Human tumour cell lines established in vitro from tumours after long-term passage as nude mouse xenografts. Comparative fingerprinting of their concanavalin-A acceptor glycoproteins
Open Access
- 1 May 1985
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 51 (5), 675-680
- https://doi.org/10.1038/bjc.1985.102
Abstract
Two human colon cancer xenografts (EC and AC) were established in tissue culture only after long-term passage in nude mice. Earlier attempts to establish cell lines were unsuccessful. The epithelioid cells retain their tumourigenicity after in vitro growth, giving rise to tumours with a take rate of 60-80%. After reimplantation, the xenografts retain a similar morphology to that of the original human tumours. Both cell lines show human karyology. Comparative mapping of Concanavalin-A acceptor glycoproteins provides a fingerprint characteristic of each cell line. These glycoprotein patterns are similar to those shown by HT-29, an established colon cancer cell line.Keywords
This publication has 20 references indexed in Scilit:
- In vivo induction of neoplastic growth in nude mouse connective tissue adjacent to xenografted human tumorsZeitschrift für Krebsforschung und Klinische Onkologie, 1983
- Concanavalin A acceptor glycoproteins: A new type of marker for the classification of tumour cellsBritish Journal of Cancer, 1983
- Stability of the glycoproteins from a primary human pancreatic carcinoma during cell culture and in vivo passage in nude miceBritish Journal of Cancer, 1983
- Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase: Application to arenavirus and flavivirus proteinsAnalytical Biochemistry, 1982
- The use of tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranesJournal of Immunological Methods, 1982
- Establishment and characterization of primary human pancreatic carcinoma in continuous cell culture and in nude miceBritish Journal of Cancer, 1979
- In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stainExperimental Cell Research, 1977
- Transplantation of Human Renal Cell Carcinoma to the Nude Mice: As an Intermediate of in Vivo and in Vitro StudiesJournal of Urology, 1976
- High resolution two-dimensional electrophoresis of proteins.1975
- Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinomaIn Vitro Cellular & Developmental Biology - Plant, 1974