EFFECT OF GLUCOSE AND PHOSPHATE ON THE FORMATION OF MEMBRANE-ASSOCIATED PARTICLES IN AEROBICALLY GROWN ESCHERICHIA COLI

Abstract

The density of membrane-associated particles (MAP) was examined by freeze-fracture technique on E. coli cells grown aerobically with or without glucose. The MAP density of the cells grown with glucose was about 1/2 that of the cells grown without glucose. In parallel with this decrease of the MAP density, the amount of cytochrome b1 and succinate dehydrogenase activity were also reduced in the cells grown with glucose. By the addition of Pi, the MAP density and cytochrome b1 content increased simultaneously in the cells grown with glucose, although activity of succinate dehydrogenase did not increase.