Adenovirus‐mediated gene transfer to murine retinal cells in vitro and in vivo
- 27 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 347 (2-3), 117-122
- https://doi.org/10.1016/0014-5793(94)00512-5
Abstract
Adenovirus‐mediated gene transfer to retinal cells was evaluated using the replication‐defective recombinant adenovirus vector Ad2/CMVlacZ‐1 (coding for β‐galactosidase) both in an in vitro murine culture model and in vivo in adult mice. In vitro, no difference in infectability of neuronal and glial cells was observed, and 50% of neurons expressed the exogenous gene at low viral concentration (10 pfu/cell). In vivo, intraocular injection of 3 × 106 pfu Ad2/CMVlacZ‐1 resulted in expression of the transferred β‐galactosidase gene in retinal pigment epithelium and ganglion cells. These results demonstrate that Ad2/CMVlacZ‐1 is an effective vector for gene transfer into retinal cells.This publication has 18 references indexed in Scilit:
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