Quantitation Using Enhanced Signal Tags: A Technique for Comparative Proteomics
- 16 October 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 2 (1), 15-21
- https://doi.org/10.1021/pr0255506
Abstract
Differential amidination of N-termini and lysine residues provides the basis for a novel approach to protein quantitation using MALDI mass spectrometry. Because the amidination of lysines increases their basicity and therefore MALDI ionization yields, the method is called quantitation using enhanced signal tags (QUEST). Amidine labels differ by methylene groups, leading to 14 Da mass differentials. The utility of QUEST is demonstrated while analyzing the digests of two model proteins using MALDI-TOF mass spectrometry. Keywords: comparative proteomics • MALDI • quantitation • QUEST • amidination • derivatizations • mass spectrometry • enhanced ionizationKeywords
This publication has 14 references indexed in Scilit:
- Optimization of Guanidination Procedures for MALDI Mass MappingAnalytical Chemistry, 2002
- Comparative proteomics based on stable isotope labeling and affinity selectionJournal of Mass Spectrometry, 2002
- Global internal standard technology for comparative proteomicsJournal of Chromatography A, 2002
- Differential stable isotope labeling of peptides for quantitation and de novo sequence derivationRapid Communications in Mass Spectrometry, 2001
- A combination of chemical derivatisation and improved bioinformatic tools optimises protein identification for proteomicsElectrophoresis, 2001
- Enhancing the intensities of lysine-terminated tryptic peptide ions in matrix-assisted laser desorption/ionization mass spectrometryRapid Communications in Mass Spectrometry, 2000
- Improved matrix-assisted laser desorption/ionization mass spectrometric analysis of tryptic hydrolysates of proteins following guanidination of lysine-containing peptidesRapid Communications in Mass Spectrometry, 2000
- Probability-based protein identification by searching sequence databases using mass spectrometry dataElectrophoresis, 1999
- Proteomics and automationElectrophoresis, 1999
- Mass SpectrometryAnalytical Chemistry, 1998