Multiple genes coding for octopine-degrading enzymes in Agrobacterium
- 1 December 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 136 (3), 909-915
- https://doi.org/10.1128/jb.136.3.909-915.1978
Abstract
Most biotype 2 strains of A. tumefaciens and A. radiobacter which utilize nopaline also degrade octopine. In all such strains studied, the ability to degrade octopine did not appear to be transferred to plasmidless recipient cells under conditios of plasmid transfer in which the ability to utilize nopaline was transferred. An octopine-degrading mutant was isolated in a strain cured of its plasmid, suggesting that genes of octopine degradation may have a chromosomal location in some strains. In strains in which octopine utilization is coded by plasmid genes, octopine degradation was always inducible, but in strains which degrade both octopine and nopaline, octopine utilization was constitutive although nopaline degradation was inducible. When plasmids coding for octopine-utilizing ability were transformed into a strain containing either a nopaline- or null-type plasmid, transformants able to degrade octopine were either not observed or were unstable upon purification. Plasmids associated with virulence are probably incompatible with one another, and therefore imply that the major groups of plasmids associated with virulence have a common origin.This publication has 22 references indexed in Scilit:
- Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciensPlasmid, 1978
- Inducible Permease Involved in the Uptake of Octopine, Lysopine and Octopinic Acid by Agrobacterium tumefaciens Strains Carrying Virulence-associated PlasmidsJournal of General Microbiology, 1977
- Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesisCell, 1977
- A useful synthesis of nopaline, a crown gall tumor metaboliteBiochemical and Biophysical Research Communications, 1977
- Chromosomal recombination and mapping in Rhizobium leguminosarumNature, 1976
- Isolation and Characterization of Agrobacterium tumefaciens Mutants Affected in the Utilization of Octopine, Octopinic Acid and LysopineJournal of General Microbiology, 1976
- Agrobacterium: correlations between and transfer of pathogenicity, octopine and nopaline metabolism and bacteriocin 84 sensitivityPhysiological Plant Pathology, 1976
- Octopine déshydrogénase Purification et propriétés catalytiquesBiochimica et Biophysica Acta (BBA) - Enzymology, 1969
- Transfer of Virulence between Isolates of AgrobacteriumNature, 1969
- Studies on Diastereoisomeric α-Amino Acids and Corresponding α-Hydroxy Acids. VIII. Configuration of the Isomeric OctopinesJournal of the American Chemical Society, 1957