Angiotensin II in epitheloid (Renin containing) cells of rat kidney
- 1 January 1981
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 72 (4), 499-509
- https://doi.org/10.1007/bf00493271
Abstract
The PAP-method was used for immunocytochemical investigations with antisera against angiotensin (ang) I, ang II and renin in kidneys of rats and mice. In 14 rats, ang II was found in the media of the afferent arteriole — both in the region of the JGA and upstream until the interlobular artery. Serial sections alternately reacted for ang II and renin revealed that the octapeptide is contained in the well known renin positive epitheloid cells of the afferent arteriole and, beyond that, together with renin probably in the same “specific” granules. Fixation conditions were critical for the visualization of immunoreactivity. With ang I antisera, comparable in terms of titer and affinity to the ang II antisera, specific immunoreactivity could not be found in the kidneys of rats. With horse radish peroxidase and ferritin as tracers it could be shown that the epitheloid cells of the JGA have the ability to pinocytize and incorporate macromolecules into their granules. It is suggested that ang II is taken up by these cells through the same route. Intracellular generation of ang II appears unlikely as an explanation. Functionally the selective uptake of ang II by epitheloid cells might be a specific process, possibly connected with the negative feedback of the octapeptide on renin secretion. Negative results in mice may be explained by a small uptake or more rapid degradation of ang II by the epitheloid cells.Keywords
This publication has 26 references indexed in Scilit:
- The intrarenal renin-angiotensin-systemKlinische Wochenschrift, 1982
- An angiotensin-like peptide in the brainLife Sciences, 1980
- Evidence for mesangial glomerular receptors for angiotensin II linked to mesangial cell contractilityFEBS Letters, 1980
- Immunocytochemical localization of renin in mouse kidneyHistochemistry and Cell Biology, 1979
- Angiotensin-Converting Enzyme: Vascular Endothelial LocalizationScience, 1976
- Angiotensin II: Renal localization in glomerular mesangial cells by autoradiographyKidney International, 1975
- Purification of rabbit angiotensin II-antibodies by affinity chromatographyCellular and Molecular Life Sciences, 1975
- Evidence for renal glomerular receptors for angiotensin IIKidney International, 1974
- Enzyme activities of the single juxtaglomerular apparatus in the rat kidneyKidney International, 1972
- Relations of the centrolobular region of the glomerulus to the juxtaglomerular apparatusJournal of Ultrastructure Research, 1962