Effect of ACTH on Protein Synthesis in Isolated Perfused Beef Adrenal Gland.

Abstract

Isolated beef adrenal glands were perfused with homologous blood containing [epsilon]-C14-DL-lysine and a nutritionally complete amino acid mixture. The gland protein was analyzed for C14 activity. The perfusate was consistent in promoting gland protein synthesis in addition to an elevated corticosteroid output as compared to levels before ACTH addition. These effects were not observed with a different preparation of ACTH. Incorporation of C14 into the plasma proteins was not significant. Relationship of the enhanced protein synthesis in the adrenal glands to the enzyme proteins involved in the biosynthesis of the adrenal cortical steroids is discussed. The possibility that increased protein synthesis is at least partially responsible for the adrenal hypertrophy observed in vivo after ACTH administration is discussed.