Radioimmunoassay of the anabolic agent zeranol. II. Zeranol concentrations in urine of sheep and cattle implanted with zeranol (Ralgro)

Abstract

A radioimmunoassay for zeranol was validated and used to measure the concentration of zeranol in the urine of sheep and cattle treated with zeranol (Ralgro). The assay uses an antibody raised against zeranol-16-carboxyl-propyl ether conjugated to human serum albumin. In sheep and cattle urine the limits of detection were .apprx. 2 and 2.5 ng/ml, respectively. In 2 trials 13 sheep were implanted with 12 mg zeranol at the base of the ear. The mean maximum concentrations of zeranol observed in urine were 45 ng/ml (trial I) on day 35 and 90 ng/ml (trial II) on day 56, and had declined to 26 ng/ml 42 days after implantation (trial I) and 11.7 ng/ml 70 days after implantation (trial II). In 4 cattle implanted with 36 mg zeranol the concentrations of zeranol in urine reached a mean maximum concentration of 13.5 ng/ml 22 days after implantation and had declined to 2.9 ng/ml 69 days after implantation.