A polyene antibiotic Nystatin injected into the subcutaneous tissues of the rat’s hind-paw provokes a long-lasting localized inflammation. This inflammationis prevented or antagonized by steroidal and non-steroidal anti-inflammatory drugs administered respectively before or after inflammation induction. Among the inhibitors of protein synthesis tested cyclophosphamide, alanosine and 6-mercaptopurine inhibit the development of Nystatin inflammation.