A Modified Technique for the Microscopic Examination of the Xylem of Whole Plants or Plant Organs

Abstract

A modification of a clearing technique for uncut material is described together with the subsequent staining and permanent mounting. The object of such treatment is the elucidation of the course and structure of the xylem in whole plants or plant organs. The method has been successfully employed with a variety of vascular plants. It involves (1) clearing in 70-75% lactic acid at 58-60[degree] C; (2) bleaching in Eau de Javelle at 40[degree] C; (3) staining the xylem with ammoniacal fuchsin; (4) mounting in euparol or Canada balsam.