Pulsatile flow filtration of yeast cell debris: Influence of preincubation on performance

Abstract

The recovery of proteins from yeast cell debris suspension was investigated using a vortex mixing technique based on the combination of oscillatory flow and a baffled flat-sheet microfiltration system. For this system, increased protein transmission was obtained through the use of low transmembrane pressures and the preincubation of the yeast cell debris feed suspension at 30°C. Furthermore, a plateau in the flux-time curve was observed. In the absence of baffles and pulsations, preincubation had a little effect. © 1993 Wiley & Sons, Inc.