Inhibition of Virus Release by Antibodies to Surface Antigens of Influenza Viruses

Abstract

When influenza virus was mixed with antisera to its surface subunits before inoculation of cell cultures, anti-hemagglutinin antibodies neutralized infectivity but anti-neuraminidase did not. When the antisera were added after infection of cell cultures, anti-hemagglutinin and anti-neuraminidase antibodies were equally effective in reducing virus titers in culture fluids. Decreased virus titers were not due to interference of antibody with assay and were not accompanied by a reduction in the synthesis of hemagglutinin and neuraminidase subunits. Both antisera also effectively prevented in vitro virus spread. Inhibition of virus release by neuraminidase antibody appeared unrelated to its antienzyme property. Hydrolysis of N-acetyl neuraminic acid residues of infected host cells proceeded unimpaired in the presence of subunit antisera. Anti-hemagglutinin and anti-neuraminidase antibodies may act to prevent virus release by binding newly formed virus subunits to each other and to anti-genically altered cell membranes.