THE CHEMICAL FRACTIONATION OF AN ADRENOCORTICOLYTIC DRUG123

Abstract
It has long been known that technical DDD (2,2-bis (parachlorophenyl) 1,1-dichloroethane) produces adrenal cortical atrophy, but the actual active agent has not been isolated. To isolate this agent, technical DDD was fractionated by recrystallization, oxidation, column chromatography, and chemical and physical analysis into several fractions. Most of the material (83%) was p,p''-DD, 10% was o,p''-DDD, and the remainder consisted of unidentified materials.