Mammalian reticulocytes lose adhesion to fibronectin during maturation to erythrocytes.
- 1 January 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (2), 440-444
- https://doi.org/10.1073/pnas.82.2.440
Abstract
Three situations in which a large fraction of circulating red blood cells [RBC] attach tightly and specifically to fibronectin rabbits made anemic by repeated bleeding, patients with hemolytic anemia and functional asplenia and splenectomized normal humans and splenectomized mice are described. Upon induction of anemia in rabbits, the proportion of circulating RBC capable of specifically attaching to fibronectin-coated plastic increased in parallel with the number of reticulocytes. Fibronectin-adherent red cells were barely detectable when the rabbit had recovered from the anemia. Attachment of reticulocytes to fibronectin was specific; cells did not attach to dishes coated with albumin, laminin, or collagen. None of these proteins promoted the attachment of normal erythrocytes. About 75% of the erythrocytes from splenectomized mice (but not control mice) also attached specifically to fibronectin 40 days after surgery. The effect of splenectomy was incomplete and transient; adherent cells were not detectable 8 wk after splenectomy. As judged by labeling studies with [35S]methionine, newly emergent reticulocytes preferentially attached to fibronectin. About half of the reticulocytes in erythropoietically unstressed mice lose their ability to attach to fibronectin, possibly due to loss of fibronectin-adhesive components, during passage through the spleen. The others loss their ability to interact with fibronectin before release, in the bone marrow, or in some extrasplenic site.This publication has 23 references indexed in Scilit:
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